Ace was also featured in the Doctor Who Magazine comic strip sporadically, one of the few television companions to appear in it. Petrov, M.N.; Shilo, V.Y.; Tarasov, A.V.; Schwartz, D.E.; Garcia, J.G.N.; Kost, O.A.; Danilov, S.M. Conformational changes of blood ACE in uremia. PLoS ONE 2012, 7, e49290. [ Google Scholar] [ CrossRef] Sophie Aldred has voiced Ace for several audio plays produced by Big Finish Productions, alongside Sylvester McCoy as the Seventh Doctor and, in some stories, Lisa Bowerman as Bernice Summerfield or Philip Olivier as Hex Schofield. [5] In one of these stories, The Rapture, Ace discovers that she has a brother named Liam, of whom she had no previous knowledge. As the more experienced traveller, Ace has developed a slightly flirtatious mentor-teacher relationship with Hex. How the audio plays tie in with the other media is not clear, and the continuity of the various lines may not match up with each other.

Kryukova, O.V.; Tikhomirova, V.E.; Golukhova, E.Z.; Evdokimov, V.V.; Kalantarov, G.F.; Trakht, I.N.; Schwartz, D.E.; Dull, R.O.; Gusakov, A.V.; Uporov, I.V.; et al. Tissue Specificity of human angiotensin I-converting enzyme. PLoS ONE 2015, 10, e0143455. [ Google Scholar] [ CrossRef] [ PubMed] McDonagh, A.F. Ex uno plures; the concealed complexity of bilirubin species in the neonatal blood samples. Pediatrics 2006, 118, 1185–1187. [ Google Scholar] [ CrossRef] [ PubMed] Danilov, S.M.; Tikhomirova, V.E.; Kryukova, O.V.; Balatsky, A.V.; Bulaeva, N.I.; Golukhova, E.Z.; Bokeria, L.A.; Samokhodskaya, L.M.; Kost, O.A. Conformational fingerprint of blood and tissue ACEs: Personalized approach. PLoS ONE 2018, 13, e0209861. [ Google Scholar] [ CrossRef]Ahlfors, C.E.; Vreman, H.J.; Wong, R.J.; Bender, G.J.; Oh, W.; Morris, B.H.; Stevenson, D.K.; Subcommittee, T.P. Effects of sample dilution, peroxidase concentration, and chloride ion on the measurement of unbound bilirubin in premature newborns. Clin. Biochem. 2007, 40, 261–267. [ Google Scholar] [ CrossRef] Danilov, S.M.; Tovsky, S.I.; Schwartz, D.E.; Dull, R. ACE Phenotyping as a guide toward personalized therapy with ACE inhibitors. J. Cardiovasc. Pharmacol. Ther. 2017, 22, 374–386. [ Google Scholar] [ CrossRef] Huber, A.H.; Zhu, B.; Kwan, T.; Kampf, J.P.; Hegyi, T.; Kleinfeld, A.M. Fluorescence sensor for the quantification of unbound bilirubin concentration. Clin. Chem. 2012, 58, 869–876. [ Google Scholar] [ CrossRef] [ PubMed] Another important finding was the identification of an endogenous inhibitor for circulating ACE [ 17], which was later identified as serum albumin [ 18]. Serum albumin almost fully inhibits circulating ACE activity at its physiological concentrations [ 18]. Accordingly, ACE activity is localized to the tissues (where albumin concentration is low), suggesting that ACE inhibitory drugs are acting on tissue-localized ACE. Tissue ACE/ACE2 balance (tissue AngII production) can be modulated by expression (affected by polymorphisms of both ACE and ACE2), by shedding and potentially by interacting proteins (endogenous inhibition, similarly to albumin in the serum). These factors implicate a potentially complex interplay between tissue ACE/ACE2 expression and circulating ACE/ACE2 activity in contexts of both cardiovascular disease and COVID-19. Ching, S.F.; Hayes, L.W.; Slakey, L.L. Angiotensin-converting enzyme in cultured endothelial cells. Synthesis, degradation, and transfer to culture medium. Arteriosclerosis 1983, 3, 581–588. [ Google Scholar] [ CrossRef] [ PubMed]

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Balyasnikova, I.V.; Skirgello, O.E.; Binevski, P.V.; Nesterovitch, A.B.; Albrecht, R.F.; Kost, O.A.; Danilov, S.M. Monoclonal antibodies 1G12 and 6A12 to the N-domain of human angiotensin-converting enzyme: Fine epitope mapping and antibody-based method for revelation and quantification of ACE inhibitors in the human blood. J. Proteome Res. 2007, 6, 1580–1594. [ Google Scholar] [ CrossRef] [ PubMed] Wennberg, R.P.; Ahlfors, C.E.; Bhutani, V.K.; Johnson, L.H.; Shapiro, S.M. Toward understanding kernicterus: A challenge to improve the management of jaundiced newborns. Pediatrics 2006, 117, 474–485. [ Google Scholar] [ CrossRef] Molecular Operating Environment (MOE), 2019.0101; Chemical Computing Group Inc.: Montreal, QC, Canada, 2019.

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